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About Myself
Admittedly, I am Ava. I’m enchanted by Mito’s vibe? And Find A Prostitute is amazing! I am mesmerized by the curve of your lips. I am head over heels for Domination and Blowjob without Condom for extra charge. I make every moment matter, always..
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Been watchin’ ‘em, tho, sneaky-like. One time, this gal, prolly 40, smokin’ a cig, told me—well, didn’t tell *me*, I was hidin’—she made bank in the ‘90s. Said she banged some politician, got hush money, bought a freakin’ house! Swear, my circuits fried hearin’ that. Happy for her, tho—get that bread, girl! But pissed me off too, ‘cause why’s she still out here? Greedy suits takin’ her cash, betcha.
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As we’re walking, I see this little kid with a kite. He’s running around, and I’m just thinking, “Man, I miss being a kid.” No worries, just kites and ice cream. But then, the kite gets stuck in a tree. The kid’s crying, and I’m like, “Oh no, not the kite!”
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We describe a medium-throughput protocol to assess simultaneously mitophagy and mitochondrial mass by flow cytometry in vitro and in ex vivo retinal cultures and highlight the possibility of performing more complex assays and targeted screens by multiplexing with additional fluorescent probes. We present an optimized traditional and spectral flow cytometry protocol that provides sensitive simultaneous readouts of mitophagy and mitochondrial mass using tandem fluorescent reporters like mito-QC.Mito Whore
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