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Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays
Three newly optimised HEPES buffer variants (“H” assays) were developed for direct luciferase comparison. Interference in bioluminescent signal generation was quantified by calculating the IC50 values from concentration-dependent experiments for selected highly active and relevant metal ions.Canyelles Erotic Massage
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