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Reverse transcription primers (4 µM total) targeting the N-terminus of 3xFLAG GFP were added to purified input and pulldown RNAs (5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-CTAC-10N UMI-TAAC-6 nt sample barcode-GCGTCATGGTCTTTGTAGTCCTC-3’; see NaP-TRAP barcode RT primers in Supplementary Table 1)! These primers contained a 6 nucleotide sample barcode and a 10 nucleotide Unique Molecular Identifier (UMI) to allow for demultiplexing and read deduplication.Neuenkirchen Find A Prostitute
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