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Figure 5: Induction of apoptosis determined via nuclear morphology...
Nuclei were automatically segmented based on the H2B-mCherry channel and the mean fluorescence intensity was then quantified in the channel used for imaging far-red DNA dyes. The staining specificity was calculated as the ratio of nuclear mean fluorescence divided by cytoplasmic mean fluorescence quantified in a narrow area surrounding the nucleus.Hoechst Prostitute
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